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Dada las peculiaridades de trabajo en este tipo de microscopía, sobre todo por trabajar en vacío, hay que tener en cuenta que cualquier muestra independientemente de su naturaleza u origen deben estar completamente deshidratas y desecadas. La visualización de muestras mal deshidratadas o desecadas será muy deficiente o prácticamente imposible, excepto en (ESEM), que sí permite la visualización de este tipo de muestras. Por otro lado, las muestras inertes metálicas que conducen bien la electricidad se pueden observar directamente al SEM, pero el resto han de ser metalizadas (cubiertas de un metal conductor). 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La principal ventaja de este tipo de microscopía es alcanzar una extraordinaria amplificación de la imagen de la muestra manteniendo un poder de resolución casi mil veces mayor que el óptico. Estas magníficas propiedades se deben a que la fuente de iluminación usada es un haz de electrones. El funcionamiento de la microscopía electrónica, sobre todo la de transmisión, es análogo al funcionamiento de un microscopio óptico. Por tanto una vez entendido los fundamentos técnicos de la microscopía óptica serán también fáciles de entender los de la microscopía electrónica y, para su descripción, nos vamos a basar inicialmente en el microscopio electrónico de transmisión. "]]]],["element",{"elementId":"38"},["name","Coverage"],["description","The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant"],["elementTextContainer",["elementText",{"elementTextId":"12604"},["text","2009"]]]],["element",{"elementId":"45"},["name","Publisher"],["description","An entity responsible for making the resource available"],["elementTextContainer",["elementText",{"elementTextId":"12605"},["text","OCW Universidad de Murcia"]]]]]]],["tagContainer",["tag",{"tagId":"4810"},["name","Microscopía electrónica de barrido"]],["tag",{"tagId":"4811"},["name","microscopio electrico."]]]],["item",{"itemId":"2519","public":"1","featured":"1"},["fileContainer",["file",{"fileId":"2441"},["src","https://bvhumanidades.usac.edu.gt/files/original/d90e9f9296d4f47fb05b6b531bdd6545.pdf"],["authentication","09fc1e9c3be72477715d59f3433adedf"]]],["collection",{"collectionId":"5"},["elementSetContainer",["elementSet",{"elementSetId":"1"},["name","Dublin Core"],["description","The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. 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For more information see, http://dublincore.org/documents/dces/."],["elementContainer",["element",{"elementId":"50"},["name","Title"],["description","A name given to the resource"],["elementTextContainer",["elementText",{"elementTextId":"12596"},["text","Manipulación de muestras para su estudio al microscopio óptico  "]]]],["element",{"elementId":"43"},["name","Identifier"],["description","An unambiguous reference to the resource within a given context"],["elementTextContainer",["elementText",{"elementTextId":"12597"},["text","http://ocw.um.es/ciencias/microscopia-aplicada-a-las-ciencias-forenses/material-de-clase"]]]],["element",{"elementId":"41"},["name","Description"],["description","An account of the resource"],["elementTextContainer",["elementText",{"elementTextId":"12598"},["text","La preparación, o manipulación, adecuada de las muestras es fundamental para llevar a cabo su correcto estudio microscópico. Una deficiente preparación de la muestra puede llevarnos a su incorrecta visualización y, por tanto, a un mal estudio e interpretación, que ni el mejor de los instrumentos microscópicos nos podría solucionar. Las técnicas para la manipulación y la preparación de las muestras son muy numerosas y cada una está orientada a conseguir unos resultados concretos; pero el tipo de muestra y lo que queramos ver en ella también es determinante en la elección de una técnica u otra. Los procedimientos para la preparación de muestras se pueden dividir en diferentes fases, o pasos técnicos, aunque, dependiendo del tipo de muestra, no todos son necesarios. Estos pasos se pueden englobar en: fijación, conservación, disección o extracción de partes, tinción, inclusión, y montaje. 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For more information see, http://dublincore.org/documents/dces/."],["elementContainer",["element",{"elementId":"50"},["name","Title"],["description","A name given to the resource"],["elementTextContainer",["elementText",{"elementTextId":"12591"},["text","Fundamentos de Microscopía Óptica "]]]],["element",{"elementId":"43"},["name","Identifier"],["description","An unambiguous reference to the resource within a given context"],["elementTextContainer",["elementText",{"elementTextId":"12592"},["text","http://ocw.um.es/ciencias/microscopia-aplicada-a-las-ciencias-forenses/material-de-clase"]]]],["element",{"elementId":"41"},["name","Description"],["description","An account of the resource"],["elementTextContainer",["elementText",{"elementTextId":"12593"},["text","El uso de determinados elementos colocados en el condensador y/o los objetivos (raramente en los oculares), que se interponen al haz luminoso filtrándolo u obstaculizándolo da lugar a los distintos tipos de microscopía óptica. Este tipo de dispositivos manipulan físicamente el haz luminoso seleccionando parte de las ondas de las que se compone dicho haz, lo que provoca una imagen contrastada de la muestra sin necesidad de teñirla. Podemos definir los siguientes tipos de microscopía (más usuales) "]]]],["element",{"elementId":"38"},["name","Coverage"],["description","The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant"],["elementTextContainer",["elementText",{"elementTextId":"12594"},["text","2009"]]]],["element",{"elementId":"45"},["name","Publisher"],["description","An entity responsible for making the resource available"],["elementTextContainer",["elementText",{"elementTextId":"12595"},["text","OCW Universidad de Murcia"]]]]]]],["tagContainer",["tag",{"tagId":"4157"},["name","Microscopía óptica"]],["tag",{"tagId":"4806"},["name","tipos de microscopio"]]]],["item",{"itemId":"2517","public":"1","featured":"1"},["fileContainer",["file",{"fileId":"2439"},["src","https://bvhumanidades.usac.edu.gt/files/original/27b0bbc3e204911fe4071f33f5870b74.pdf"],["authentication","e277d92e41c7d6a9f45ede438482d894"]]],["collection",{"collectionId":"5"},["elementSetContainer",["elementSet",{"elementSetId":"1"},["name","Dublin Core"],["description","The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. 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For more information see, http://dublincore.org/documents/dces/."],["elementContainer",["element",{"elementId":"50"},["name","Title"],["description","A name given to the resource"],["elementTextContainer",["elementText",{"elementTextId":"12571"},["text","Topología Algebraica"]]]],["element",{"elementId":"43"},["name","Identifier"],["description","An unambiguous reference to the resource within a given context"],["elementTextContainer",["elementText",{"elementTextId":"12572"},["text","https://libroslibresgratis.wordpress.com/category/libros/ciencias-generales/matematicas-ciencias-generales/page/3/"]]]],["element",{"elementId":"41"},["name","Description"],["description","An account of the resource"],["elementTextContainer",["elementText",{"elementTextId":"12573"},["text","La topología algebraica proporciona técnicas capaces de analizar en profundidad los espacios topológicos que aparecen en geometría, tanto en geometría diferencial como en geometría algebraica, y en aquellas ramas del análisis directamente relacionadas con la geometría, como el calculo diferencial e integral en variedades, o la teoría de funciones de variable compleja y —especialmente dentro de ésta— en la teoría de las superficies de Riemann. 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